摘要
目的探讨细粒棘球绦虫Eg95抗原在BCG中的表达,为囊型包虫病疫苗的开发利用提供依据。方法用热诱导方法诱导rBCG-Eg95疫苗,用SDS-PAGE和Westernblot鉴定所表达的蛋白。结果表达重组蛋白为可溶性蛋白,分子质量单位为16.5ku。Westernblot示重组蛋白具有抗原性,用薄层扫描Bio-RadQuantityone分析系统分析,表达效率为占BCG菌体总蛋白的12%。结论细粒棘球绦虫Eg95抗原在BCG中成功表达,为BCG发展为多价疫苗载体奠定了基础。
Objective To study the expression of the Echinococcus granulosus 95 （Eg95） antigen encoding gene in BCG. Methods Recombinant BCG-Eg95 vaccine was induced daily at 45℃ for 45 rain at the last there days, The product from BCG was analyzed by SDS-PAGE and Western blot, Results The expressed recombinant Eg95 was soluble and could be detected by SDS-PAGE about the molecular weight of 16.5 ku, and possessed antigenicity in Western blot . The expression efficiency of Eg95 was about 8 of the total bacterial protein in BCG, Conclusion The E. granulosus 95 antigen gene in BCG is successfully expressed. The study could provide basis to develop the BCG multivalent vaccine vectors.
出处
《中国病原生物学杂志》
CSCD
2006年第2期95-97,共3页
Journal of Pathogen Biology
基金
国家自然科学基金(No.30200239)
重庆市高等学校优秀中青年骨干教师资助项目(No.G0422)。
