摘要
目的研究蜂胶总黄酮(TFP)对自发性糖尿病大鼠糖脂代谢的影响。方法将50只自发性糖尿病大鼠按血糖值随机分为对照组、消渴丸组(2 000 mg·kg-1)、蜂胶总黄酮高剂量组(240 mg·kg-1)、中剂量组(120 mg·kg-1)、低剂量组(60mg·kg-1)。各组大鼠每天灌胃给药1次,连续8周,对照组灌胃同体积羧甲基纤维素钠溶液。末次给药结束后,禁食12 h,乙醚麻醉,腹主动脉取血,检测自发性糖尿病大鼠血清中血糖(Glu)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(GHb)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)、胰岛素(INS)、C肽(C-P)、肿瘤坏死因子(TNF-α)、游离脂肪酸(FFA)、一氧化氮(NO)及肝糖原水平。结果与对照组比较,蜂胶总黄酮高、中、低剂量组血糖、甘油三酯、总胆固醇、低密度脂蛋白胆固醇、丙二醛及肿瘤坏死因子-α水平明显降低(P<0.05或P<0.01或P<0.001),高密度脂蛋白胆固醇、超氧化物歧化酶、胰岛素、C肽及肝糖原含量明显升高(P<0.05或P<0.01或P<0.001);蜂胶总黄酮高、中剂量组糖化血红蛋白及游离脂肪酸水平明显降低(P<0.05或P<0.01或P<0.001),谷胱甘肽及一氧化氮含量明显升高(P<0.05或P<0.01),低剂量组则无显著性差异(P>0.05)。消渴丸组超氧化物歧化酶、肝糖原、一氧化氮及C肽明显升高(P<0.05或P<0.01),血糖、丙二醛、甘油三酯及糖化血红蛋白水平明显降低(P<0.05或P<0.01或P<0.001),对其他指标则无明显影响(P>0.05)。结论蜂胶总黄酮可明显降低自发性糖尿病大鼠血糖水平,明显改善自发性糖尿病大鼠糖脂代谢及抑制胰岛素抵抗。
OBJECTIVE To study on glucolipid metabolism effect of the total flavones of propolis （TFP） in Goto-Kakisaki rats （ GK Rats） . METHODS Fifty GK rats were randomly divided into 5 groups by blood glucose, the model group, positive drug group （2 000mg·kg^-1） and the high（240 mg·kg^-1） dose of the TFP group, the medium（ 120 mg·kg^-1） doses of the TFP group, low （60 mg·kg^-1 ） doses of the TFP group. The GK rats in TFP groups were given with TFP by intragastric administration, continuously treated for 8 weeks, the control group were given equal sodium carboxymethylcellulose （CMC-Na） and positive drug group were given （2 000 mg·kg^-1） dose of Xiaoke pills by intragastric administration. The glucose （ Glu）, total cholesterol （ TC ）, triglyceride （ TG）, high density lipoprotein（ HDL-C）, low density lipoprotein（ LDL-C）, glycated hemoglobin（ GHb）, superoxide dismutase （SOD）, malondialdehyde （MDA） , glutathione （GSH） , serum insulin （INS） , C-peptide （C-P） , tumor necrosis factor-α （TNF-α） free fatty acid （ FFA）, nitric oxide （ NO）, and hepatic glycogen were measured at the end of the experiment. RESULTS Compared with the control group, the levels of GLU, TG, TC, LDL-C, GHb, MDA and TNF-α were decreased significantly （ P 〈 0. 05 or P 〈 0. 01 or P 〈 0. 001 ）, the levels of HDL-C,SOD, INS, hepatic glycogen and C-P were increased significantly in the high-dose group, medium-dose group and low-dose group（P 〈0. 05 or P 〈0. 01 or P 〈0. 001 ）. The levels of GHb and FFA were decreased significantly（P 〈0. 05 or P 〈0. 01 or P 〈0. 001 ） , the levels of GSH and NO were increased significantly（P 〈0. 05 or P 〈0. 01 ） in the high-dose group and medium-dose group, the other indexes had no significant difference （P 〉 0. 05 ） in the low-dose group. The levels of SOD, hepatic glycogen, NO and C-P were increased significantly（P 〈0. 05 or P 〈0.01 ）, the levels of GLU, MDA, TG and GHb were decreased significantly（P 〈 0. 05 or P 〈 0. 01 or P 〈 0. 001 ） in Xiaoke pills group. CONCLUSION TFP can significantly decrease the level of blood glucose, significantly improve the glucose and lipid metabolism and inhibit insulin resistance in deficiency of GK rats.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2015年第3期217-220,共4页
Chinese Pharmaceutical Journal
基金
国家"十一五"重大新药创制(2009ZX09103-418)
吉林省科技支撑计划(20110946)
关键词
蜂胶总黄酮
糖尿病
自发性糖尿病大鼠
糖脂代谢
胰岛素抵抗
total flavones of propolis
diabetes mellitus
GK rats
glucolipid metabolism
insulin resistance
