摘要
目的 :探讨丹参对肢体缺血再灌注损伤的影响。方法 :2 0只大白兔随机分成两组 ,于兔的双侧大腿部持续上止血带 3小时。A组 10只 ,在松止血带前后 10分钟分别给予丹参 2ml/kg (1 5g/ml) ;B组 10只 ,在同一时间给予等容量的生理盐水。在松止血带前及松后 30分钟、 1、 3、 6小时分别采取血样本来测定多核粒细胞(PMN)计数、白蛋白含量及丙二醛 (MDA)含量。结果 :与再灌注前相比所有的实验动物再灌注后血中PMN计数及白蛋白水平在不同的时间段有不同程度的降低 ,MDA水平则升高。所有的数据经方差分析 ,P <0 0 1。两组间进行比较 ,再灌注后 1、 3、 6小时B组血中PMN计数明显低于A组 ;再灌注后 3、 6小时B组白蛋白水平也明显低于A组 ;而血中MDA水平B组在再灌注后各个时点均高于A组。所有数据经T检验 ,P <0 0 5～ 0 0 1。结论 :丹参有减轻肢体缺血再灌注损伤的作用。
Objective:To investigate the influence of Danshen on ischemic-reperfusion injury of limb.Methods:20 rabbits were randomly divided into two groups.Tourniquet was performed on bihindlimb for 3 hours.In group A(n=10),the Danshen 2ml/kg(1.5g/ml)was given intravenously before and after releasing tourniquet 10 min.In group B(n=10),all rabbits were given same volume saline at same times.Before releasing tourniquet and after reperfusing 0.5?1?3?6 hours,the blood samples were collected for checking PMN count?albumin level and MDA level.Results:Comparing the data between the tourniquet releasing before and after,the PMN count and albumin levels decrease in different degreen on different times,and MDA levels increase.All data was treated by F-test(P<0.01).Comparing the group A with the group B,the PMN count in group B was lower than that in group A after reperfusion 3?6 hours;the MDA level was higher in group B than that in group A after reperfusion.All data was treated by T-test(P<0.05～0.01).Conclusion:Danshen may decrease the injured degree of ischemic-reperfusion injury of limb.
出处
《华西医学》
CAS
2002年第2期238-239,共2页
West China Medical Journal
