摘要
目的 :观察脑缺血再灌注过程中兴奋性氨基酸 (Excitatory amino acid,EAA)、氧自由基的变化 ,研究探索脑缺血再灌注损伤的机制。方法 :测定假手术组、缺血 30 min再灌注 60 min生理盐水 (NS)处理组和单唾液酸四已糖神经节苷脂 (GM1 ,1 0 mg/ kg,IP)处理组 ,鼠脑海马组织 EAA、丙二醛 (Malondiadehyde,MDA)的含量。实验应用全脑缺血 (4 VO)模型 ,EAA采用 HPLC法 ,MDA采用 TBA法。结果 :脑缺血再灌注 NS处理组海马组织 EAA含量明显较假手术组降低 (P<0 .0 1 ) ,MDA含量则显著性升高 (P<0 .0 1 ) ,GM1 处理组 EAA,MDA含量均无明显变化 (P>0 .0 5)。结论 :EAA。
Objective :To ovserve the excitatory amino acid(EAA), oxygen free radicals change on cerebral ischemic reperfusion ,and study the mechanism of cerebral ischemic reperfusion damage.Methods:The hippocampus EAA,malondiadehyde (MDA) contents of sham operated group,ischemia 30 min reperfusion 60 min normal sodium (NS) and GM 1treated group GM 1,10mg/kg,IP) were measured,the global cerebral ischemic reperfusion model (4VO) was made on the experiment,EAA was measured by high performance liquid chromatogrphic detemination (HPLC),MDA was measured by TBA. Results:Compared with sham operated group,the hippocampus EAA content of cerebral ischemic reperfusion NStreated group decreases significantly (P<0.01),MDA content increases significantly (P<0.01),the hippocampus EAA and MDA contents of GM 1treated group don't change significantly (P>0.05). Conclusion:EAA,oxygen free radicals are closely related with the mechanism of cerebral ischemic reperfusion damage.
出处
《卒中与神经疾病》
2000年第2期98-100,共3页
Stroke and Nervous Diseases
关键词
兴奋性氨基酸
氧自由基
缺血再灌注脑损伤
Excitatory amino acid Oxygen free radicals Cerebral ischemic reperfusion damage
