摘要
目的 探讨各种细胞因子对Ｔ细胞生长激素（ＧＨ）基因表达的影响。方法 构建含人ＧＨ调控序列的荧光素酶报告基因质粒 ＰＧＬ２－ＧＨ－ｌｕｃ，然后转染入Ｔ淋巴细胞系 Ｊｕｒｋａｔ Ｅ６－１细胞中，在培养液中分别加入各种细胞因子。结果 生理浓度的ＩＬ－１β、ＴＮＦ－β和 ＩＦＮ－γ，对 Ｊｕｒｋａｔ细胞中荧光素酶的表达具有抑制作用（Ｐ＜０.０５）。结论 细胞因子参与了调节淋巴细胞 ＧＨ基因的表达。
Aim To explore the effects of cytokines on expression of growth hormone (GH) gene in T lymphocytes. Methods The report- er gene plasmid of pGL2- GH- luc luciferase with human GH regu- lation sequence was constructed, and then was transfected into Jur- kat cells. Some cytokines were added in the cell culture medium. Results The expression of luciferase in lymphocytes could be inhib- ited by ILlβ, TNFβ and IFNγ under physiological dose (P <0. 05). Conclusion Some cytokines can regulate the expression of GH gene in lymphocytes
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第1期7-9,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助课题
No.39770350
