摘要
目的 :探讨生长激素 (GH)对T淋巴细胞功能的影响。方法 :构建GHcDNA表达质粒PcDNA GHcDNA ,然后将其转染入T淋巴细胞系 Jurkat细胞中 ,观察过度表达的内源GH和加入外源基因重组人GH对T淋巴细胞功能的影响。结果 :过量表达的内源GH使Jurkat细胞表达IL 2R和分泌IL 2、IFN γ分别增加 1 40倍、1 6 0倍和 1 98倍 ,而在培养液中加入抗GH血清后 ,GH的这种作用消失。另外 ,外源GH对Jurkat细胞的功能也有促进作用。结论
Objective:To study the effects of growth hormone on function of T lymphocytes.Methods:The expression plasmid PcDNA GH cDNA was constructed,then the plasmids were transfected into Jurkat cells,on the other hand,Jurkat cells were treated with different doses of rhGH,to investigate the effect of overexpression GH and rhGH on function of T lymphocytes.Results:Overexpression GH could increase the expression of IL 2R(140%) and secretion of IL 2(160%) and IFN γ(198%) in Jurkat cells after PHA activation,but these data were brought down by the treatment of GH antibody.rhGH could also enhancer the function of T lymphocytes after PHA activation.Conclusion:These results support the idea that GH is an autocrine and paracrine factor,and can regulate the function of T lymphocytes.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2000年第9期480-484,共5页
Chinese Journal of Immunology
基金
国家自然科学基金!(39770 35 0 )
