摘要
目的探讨影响淋巴细胞培养生长因素及去除方法。方法在正常淋巴细胞培养中去掉原培养液,加入灭菌生理水(内含有青霉素钠100 IU/mL)洗涤后再加入培养基继续培养,常规收获细胞及常规G显带油镜观察分析。结果用上述方法洗涤后收获的细胞较正常培养收获的细胞转化率、有丝分裂指数、分裂相质量均提高非常显著(P<0.01)。结论该方法可去除影响淋巴细胞培养生长的因素,保证收获细胞数量和质量,从而提高分析诊断水平,且易于推广应用。
Objective To probe into the influential factor for lymphocyte growth and its removing method. Methods The original culture liquid in normal lymphocyte medium was removed and washed with the disinfectant physiological saline （containing 100 IU/mL of benzylpenieillin sodium）. After that, the new culture medium was added for continuous cultivation. The lymphocytes had been collected conventionally and G banding analysis under immersion objective was performed. Results After the aforesaid washing method was used, cell transformation efficiency, mitotic index and quality of mitotic phase of lymphocytes were obviously improved compared with those cultivated by conventional cultivation method （P（0.01）. Conclusion It is proved that this new method can remove the influence on lymphocyte growth during cultivation and guarantee the harvesting cell number and quality, and then improve the levels of analysis and diagnosis. And also it is easy for popularization.
出处
《国际检验医学杂志》
CAS
2008年第8期689-690,共2页
International Journal of Laboratory Medicine
关键词
淋巴细胞培养
生长因素
方法
转化率
分裂相
Lymphocute cultivation
Growth factor
Method
Transformation ratio Mitotic phase
