摘要
目的：克隆和鉴定人淋巴细胞免疫反应性生长激素（ｉｒＧＨ）基因调控序列的核苷酸顺序。方法：通过ＰＣＲ扩增出人淋巴细胞ｉｒＧＨ基因５′近端的调控序列，然后将其与质粒ＰＧＥＭ７Ｚｆ（＋）连接，经转化蓝白斑筛选、酶切鉴定，获得ＰＧＥＭ７Ｚｆ（＋）ｉｒＧＨＤＮＡ（－４８４＋２ｂｐ）重组质粒，进行序列测定。结果：显示ｉｒＧＨ基因５′近端的调控序列与垂体细胞ＧＨ基因的５′近端调控序列的核苷酸顺序完全相同。
Objective:Cloning and nucleotide sequencing of the 5′ flanking region of immunoreactive growth hormone gene of human 1ymphocyte.Methods:The immunoreactive growth hormone(irGH) DNA fragments were obtained by PCR with normal human peripheral blood lymphocyte DNA as template.Then,the plasmids of PGEM7Zf(+) irGH DNA(-484 +2 bp) were constructed by ligation of irGH DNA with PGEM7Zf(+),transformation,blue/white color selection and restriction enzyme analysis.The 5′ flanking region of irGH were sequenced by using sanger's dideoxynucleitide chain temination reaction.Results:It was demonstrated that the sequence data of 5′ flanking region(-484 +2 bp) in irGH gene was identical to that of pituitary GH gene.Conclusion:The results indicate a close relationship between immune system and neuroencrine system.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第1期32-34,共3页
Chinese Journal of Immunology
基金
国家自然科学基金
