摘要
目的建立复方丹参滴丸中人参皂苷Rg1和Rb1,三七皂苷R1的含量测定方法。方法应用高效液相色谱-蒸发光散射检测法(HPLC-ELSD),采用固相萃取技术对样品进行预处理,HypersilNH2柱,流动相为乙腈-异丙醇-10mmol/L醋酸铵水溶液(冰醋酸调ph5.0)(15∶4∶1);流速:0.6mL·min-1,测定了复方丹参滴丸中人参皂苷Rg1、Rb1和三七皂苷R1的含量。结果该方法测定人参皂苷Rg1、Rb1和三七皂苷R1的线性范围为1.0～10.0μg,其加样回收率为95.3～100.4%,日内精密度≤2%、日间精密度≤4%。结论方法简便准确,可为三七制剂的含量测定方法提供借鉴。
Objective: To establish a method for determining the contents of ginsenoside Rg1 and Rb1, notoginsenoside Rg1 in compound Danshen dropping pill by HPLC/ELSD. Methods: Ginsenoside Rg1 and Rb1, notoginsenoside R1 were pretreated with solid phase extraction(SPE). The extraction conditions were as follow: Hypersit NH2 column, acetonitrile-isopropanol-10mmol/L ammonium acetate( 15∶4∶1 ) as mobile phase (iced acetic acid adjusting pH as 5.0) and the flow rate at 0.6 mL/min. Results: The linear range was from 1.0 to 10.0μg for ginsenoside Rg1 and Rb1, and notoginsenoside Rg1. The average recovery was 95.3%～100.4%.The inter-day RSD and intra-day RSD were less than 2%and 4%respectively. Conclusion: The method is concise and accurate, and will be helpful for the quality control of Radix Notoginseng and its preparations.
出处
《中药新药与临床药理》
CAS
CSCD
2002年第3期174-176,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金项目(39770909)。
关键词
复方丹参滴丸
人参皂苷
化学分析
三七皂苷
高压液相色谱法
蒸发光散射检测器
Compound Danshen Dropping Pill/Chemistry
Ginsenoside/Analysis
Notoginsenoside/Analysis
Chromatography, High Pressure Liquid
@ ELSD
