摘要
目的 建立复方丹参方中三七皂苷类成分的含量测定方法,并初步考察复方丹参方溶液的稳定性。方法采用高效液相色谱法,分别以乙腈-0.05％磷酸(19:81)为流动相,于203 nm处检测三七皂苷R1、人参皂苷Rg1、人参皂苷Re;以乙腈-水(33:67)为流动相,于203 nm处检测人参皂苷Rb1。结果 三七皂苷R1进样量在0.24～3.6μg范围时,相关系数r=0.9997;人参皂苷Rg1进样量在1.2～18μg范围时,相关系数r=0.9994;人参皂苷Re进样量在0.08～1.2μg范围时,相关系数r=0.9997;人参皂苷Rb1进样量在0.25～3.72μg范围时,相关系数r=0.9998。复方丹参方溶液于4℃放置4个月后三七皂苷类成分的含量无明显变化。结论 本法简便,快速,准确,重现性好,可作为复方丹参方的质量控制标准。复方丹参方中三七皂苷类成分的稳定性良好。
Objective To develop a method for the determination of four saponins of panax notoginseng in Compound Dan-shen Prescription (CDP) by HPLC. Methods The conditions for the experiment were: determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Re with acetonitrile - 0. 05 % phosphoric acid (19 : 81) as mobile phase and the detection wave at 203nm, determination of ginsenoside Rb1 with acetonitrile - water (33 : 67) as mobile phase and the detection wave at 203nm. Results A good linearity for notoginsenoside R1 was in the range of 0. 24 ~ 3. 6μg ( r = 0. 9997), 1. 2 ~ 18μg (r = 0. 9994) for ginsenoside Rg1, 0. 08 ~ 1. 2μg(r = 0. 9997) for ginsenoside Re and 0. 25-3. 72μg (r = 0. 9998) for ginsenoside Rb1. The stability of the four saponins is well kept at 4℃ for four months. Conclusion This method is simple, rapid, accurate and with good reproducibility and can be used for the quality control of CDP.
出处
《中药新药与临床药理》
CAS
CSCD
2003年第2期112-114,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家重点基础研究规划项目(编号G199944054403)
