摘要
目的建立清脑宣窍方有效部位中人参皂苷Rg1、Rb1及三七皂苷R1的HPLC含量测定方法。方法采用Phenomenex Luna NH2色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(80∶20),流速为1.0 mL/m in,检测波长为203 nm。结果人参皂苷Rg1平均回收率为101.86%,RSD为1.68%(n=9);三七皂苷R1平均回收率为101.23%,RSD为1.28%(n=9);人参皂苷Rb1平均回收率为102.02%,RSD为1.45%(n=9)。结论该方法简便、准确,可用于清脑宣窍方有效部位中人参皂苷Rg1、Rb1及三七皂苷R13种成分的含量测定。
Object To establish a method for the quantitative determination of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in the effective fraction of Qingnaoxuanqiao Formula by HPLC. Method The determination was carried out with phenomenex Luna NH2 column（250 mm× 4.6 mm,5 μm）. CH3CN-H2O （80： 20） was taken as mobile phase at a flow rate of 1.0 mL/min, and detection wavelength was 203 nm. Result The average recoveries of ginsenoside Rg1, ginsenosideRb1 and notoginsenoside R1 were 101.86%, 102.02% and 101.23% respectively, and RSD s of the above three compounds were 1.68 % （ n = 9）, 1.45 % （ n = 9 ） and 1.28 % （ n = 9） respectively. Conclusion The method is simple and accurate, and can be used to determine the content of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in the effective fraction of Qingnaoxuanqiao Formula.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2007年第1期54-57,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
北京市教委产学研重点项目
