摘要
目的建立玛奴西汤（MNXTgranule）的质量控制标准。方法采用薄层色谱法（TLC）对制剂中藏木香、宽筋藤进行定性鉴别；采用高效液相色谱法对制剂中藏木香的成分异土木香内酯、土木香内酯进行定量测定。结果玛奴西汤中藏木香、宽筋藤经TLC鉴别，方法专属性强，土木香内酯、异土木香内酯经HPLC含量测定，方法专属性强。可鉴别藏木香、宽筋藤，阴性对照无干扰，方法专属性强。异土木香内酯在0．281～0．842μg范围内呈良好的线性关系，r=0．9998，平均回收率（n=5）为98．5％，RSD为1．14％：土木香内酯在0．232～0．696μg范围内呈良好的线性关系，r=0．9999，平均回收率（月=5）为97．4％，RSD为1．10％。结论该方法简便、准确、重复性好，可有效控制玛奴西汤的质量。
Objective To establish the quality standard for Tibetan medicine MNXT granule. Methods Inula racemosa, Tinospora sinensis were identified by TLC; Isoalantolactone and alantolactone were determined by HPLC. Results Inula racemosa, Tinospora sinensis could be identified by TLC. The concentration of isoalantolactone was linear in the range of 0.281～0.842 μg, r=0.9998, with the average recovery rate being 98.5%, RSD beingl.14%. The concentration of alantolactone was linear in the range of 0.232～0.696 μg, r=0.9999, with the average recovery rate being 97.4%, RSD being 1.10%. Conclusion The method was simple, accurate, repeatable and able to control the aualitv of Drelgaration effectively.
出处
《国际中医中药杂志》
2013年第3期228-230,共3页
International Journal of Traditional Chinese Medicine
基金
青海省科学技术厅基金项目（gA目编号：2010-N-515）
