摘要
以黄苗榆烟草为试材、摩擦接种法接种烟草花叶病毒 ,比色法测定了几种防御酶活性 ,研究壳寡糖对烟草 烟草花叶病毒这一互作系统激发产生的一系列抗病生理生化反应的可能性。试验结果初步表明 :壳寡糖处理可导致烟草叶片PO、CAT、PPO、PAL和 β 1 3葡聚糖酶活性不同程度地提高。PO同工酶酶谱分析得知喷药后接毒处理可看到 7条酶带且谱带色浓 ,酶活性强。由此初步认为壳寡糖诱导抗病毒机制与提高PO活性及其增多同工酶酶谱的酶带有关 ;分析SOD、CAT和PPO三种酶似乎与壳寡糖诱导抗病毒机制相关不大或无相关性 ;单因素的喷药和接毒多可导致几种防御酶活性的提高 ,尤其是PAL和β 1 3葡聚糖酶活性提高明显 。
The physiology and biochemistry resistance of induced by oligochitosan in tobacco to combat against TMV infection were studied. Tests on some defensive enzymes indicated that the activity of peroxidase (PO), catalase (CAT), polyphenoloxidase (PPO), phenlalanine ammonialyase (PAL) and β 1.3 glucanase in tobacco leaves increased after treatment with oligochitosan. Spraying oligochitosan could result in 7 bands of PO isoenzyme in leaves. So it was preliminarily considered that the induced resistance of oligochitosan in tobacco to resist against TMV was closely related to the activity and zymogram type of PO isoenzyme. There was no correlation between the activity of SOD, CAT or PPO and the induced resistance. Activity of PAL and β 1.3 glucanase was clearly increased after spraying oligochitosan or inoculating TMV, but the cumulative response of the two factors was not clear.
出处
《中国生物防治》
CSCD
北大核心
2002年第2期83-86,共4页
Chinese Journal of Biological Control
