摘要
对小麦、油菜苗喷施浓度为０．２％水溶性壳聚糖后，叶片几丁质酶活性明显提高，油菜和小麦酶活性分别在处理后２ｄ、６ｄ时达最大值，均比相应对照提高２倍左右，且都表现为内切几丁质酶活性。叶片细胞间的几丁质酶比活力明显高于细胞内的酶比活力。壳聚糖诱导的油菜叶片几丁质酶在体外可抑制油菜菌核病菌生长，并能降解菌丝细胞壁。
Watersolublechitosan,apolymerof1,4Dglucosaminederivedfromcrabshellchitin,wasappliedtorape(Brassicanapus)andwinterwheat(Triticumaestivum)bymeansofleafspraying.Itwasfoundthatchitinase(EC3,2,1,14)activitieswasnearthreefoldinbothofleavesofwinterwheatandrapetreatedwith02%(W/V)chitosansolutiontothatinCK(sprayingTW80).Thepeakofchitinaseactivityinrapeandwinterwheatappearedatthe2nddayandthe6thdayrespectivelyafterchitosansolutionbeingsprayed.Chitoasninducedchitinaseshowedendochitinaseactivity.Theintercellularfluidofleaveswasextractedbythemethodofvacuuminfiltrationandcentrifugation.Resultsshowedthatchitinasehadmuchhigherspecificactivityinextractofintercellularfluidthanthatinleafhomogenates,andmostofthechitosaninducedchitinaselocatedintheextracellularspace.Chitinasefromrape leavescouldinhibitthegrowthofSclerotiniasclerotiorumandlysisthecellwallofSclerotiniasclerotiorumformingoligosaccharidesinvitro.Fromthatitcouldbeelucidatedthatchitosaninducedchitinasefunctionedasadefensiveenzymeagainstpathogenicfungi.
出处
《江苏农业学报》
CSCD
北大核心
1999年第2期67-70,共4页
Jiangsu Journal of Agricultural Sciences
基金
江苏省"九五"农业科技重点攻关项目
