摘要
采用木瓜蛋白酶Papain、碱性蛋白酶Alcalase两种蛋白酶复合水解牛肉制备富含生物活性多肽、肉碱的混合液。酶解条件的优化采用响应曲面(RSM)法,先建立数学模型,然后回归分析、模型评价,最后进行验证实验。最终确定复合酶解最佳条件:犤E犦/犤S犦=1540(I.U/g蛋白质),犤EA犦/犤Ep犦=1:7.5,犤S犦=3%,pH7.0,T=55℃,水解时间t=90min,此时DH=24.67%。
Beef protein was hydrolyzed with two kinds of commercial proteolytic enzymes (Papain, Alcalase)together. Hydrolysis conditions were investigated with response surface method. When total activity of enzyme and concentration of substrate if fixed as that of papain/=1540(I.U/g protein),/=1∶7 5,=3%,hydrolysis was carried out at 55℃pH7.0 with mixed enzymes (Alcalase:Papain=0.13)for 90min,the recovery and DH can reach 24.67%.Hydrolysates which contains a large quantity of activity peptides and L-Carnitine can be made into nutrient powder by vacuum concentration and spray drying.
出处
《食品科技》
CAS
北大核心
2002年第2期17-20,共4页
Food Science and Technology
关键词
牛肉蛋白
L-肉碱
木瓜蛋白酶
碱性蛋白酶
复合酶解
水解度
响应曲面法
数学模型
beef protein, L-Carnitine,papain,alcalase,degree of hydrolysis,hydrolyzed with mixed enzyme,response surface method,math model
