摘要
目的 :探讨血府逐瘀汤对用血瘀证兔模型血清损伤内皮细胞的保护作用 ,阐明该方调节血管内皮细胞内分泌功能的作用机理。方法 :将正常组、血瘀模型组、血瘀 +中药组和高脂血症组兔血清加入同批培养的正常兔主动脉内皮细胞中 ,作用2 4h后 ,观察细胞液中ET、NO的含量和t PA、PAI及AT Ⅲ活性的变化。结果 :与正常血清组相比 ,血瘀血清组细胞液中的ET和NO含量都明显升高 ,PAI活性也明显升高 ,AT Ⅲ活性则明显降低 ,t PA活性虽有下降趋势 ,但无显著性意义 ;而血瘀 +中药血清组细胞液中的ET和NO含量以及t PA、PAI和AT Ⅲ活性都未有明显改变。结论 :血府逐瘀汤能调节血瘀证兔模型血清对内皮细胞ET/NO的释放平衡作用和降低其对抗凝、纤溶功能的影响 ,对内皮细胞起到一定的保护作用。
To investigate the protective effects of Xuefu Zhuyu (XEZY) decoction on the vascular endothelial cells injured by rabbit blood stasis syndrome (BSS) serum and its effects of improvement on the endocrine dysfunction, we used the 4 different kinds of rabbit serum (normal, BSS with absorbed XFZY, hyperlipemic) to cultured rabbit vascular endothelial cells in \%vitro\%. The concentrations of endothelin (ET), nitric oxide (NO) and the activity of tissue\|type plasminogen activator (t\|PA), plasminogen activator inhibitor (PAI), antithrombin Ⅲ(AT\|Ⅲ)in cultured supernatant were detected at 24 hour, respectively. Results show the levels of ET, NO and the activity of PAI, except for t\|PA, increased significantly in the BSS serum group, as compared with normal serum group. No significant differences of these vaso\|active substance levels in cultured supernatant between normal and BSS with XFZY decoction. These results indicate that XFZY decoction can improve the endocrine dysfunction of the endothelial cells and protect cultured endothelial cells injured by rabbit BSS serum in \%vitro.\%
出处
《中国实验方剂学杂志》
CAS
2002年第2期12-14,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金 (392 70 81 )
广东省科委自然基金(91 1 89)
关键词
血府逐瘀汤
血管
内皮
细胞培养
中药
Xuefu Zhuyu Decoction
Blood Stasis
Endothelial cells
Vaso\|active substance
