摘要
【目的】从形态学角度观察血府逐瘀汤对用血瘀证兔血清损伤的内皮细胞的保护作用。【方法】将正常组、血瘀证组、正常加中药组和血瘀证加中药组兔血清分别加入同批培养的正常兔主动脉内皮细胞中 ,用光镜和电镜观察其形态结构的变化。【结果】光镜检查表明血瘀证组内皮细胞收缩变形 ,细胞间隙增大 ,胞浆中有暗色颗粒 ;血瘀证加中药组细胞间隙增大不明显。电镜观察发现 ,与正常组和正常加中药组相比 ,血瘀证组细胞内饮液泡明显增多 ,粗面内质网数目减少并有扩张 ,线粒体细小、嵴不清 ,细胞表面仅有少量细长的微绒毛 ,末端膨大融合 ;而血瘀证加中药组粗面内质网数目较少但不扩张 ,细胞表面微绒毛未见融合。【结论】血瘀证组兔血清严重损伤体外培养的正常血管内皮细胞 ,血府逐瘀汤对用血瘀证兔血清损伤的血管内皮细胞有一定的保护作用。
Objective To observe the protective effect of Xuefu Zhuyu Decoction (XZD) on vascular endothelial cells (VEC) injured by the serum of blood_stasis_syndrome ( BSS) rabbits. Methods The morphological features of VEC cultured with norma l rabbit serum (Group A), BSS rabbit serum(Group B), the serum of normal rabbit medicated with XZD(Group C) and the serum of BSS rabbit medicated with XZD(Group D) were observed respectively under light microscope and electron microscope. Results Under light microscope, VEC was shrinked, intercellular space widene d and cytoplasm contained dark granules in Group B. The change of intercellular space was not obvious in Group D. Under electron microscope, pinocytotic vesicle s increased, rough endoplasmic reticulum (RER) decreased and dilated, mitochondr ia became smaller and indistinct and only a small amount of microvilli appeared on cell membrane with top expanded and merged in VEC of Group B as compared with Group A and Group C. Less RER but no dilatation and fewer microvilli without to p merged were found in VEC of Group B. Conclusion The serum of BSS rabbits c an injury normal VEC cultured in vitro and XZD exerts a certain protective effe ct on VEC injured by the serum of BSS rabbits.
出处
《广州中医药大学学报》
CAS
2001年第2期105-108,114,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金!(编号 :392 70 81)
广东省科委自然科学基金!(编号 :91189)
关键词
血瘀
病理生理学
血管内皮
细胞培养
血府逐瘀汤
治疗应用
BLOOD STASIS/pathophysiology
ENDOTHELIUM, VASCULAR
CELL CULTURE
XUEFU ZHUYU DECOCTION/ther.use
DISEASE MODELS, ANIMAL
RABBITS
