摘要
目的 :探讨p5 3蛋白、增殖细胞核抗原 (PCNA)在结直肠癌细胞中的表达情况与临床病理及预后的关系。方法 :应用自动化图像分析技术及SP法免疫组化 ,对 5 3例大肠癌、30例大肠腺瘤及 10例正常肠黏膜进行p5 3、PCNA的定量检测。结果 :p5 3、PCNA阳性率分别为 5 2 38%、97 73%。上述指标各参数值在正常肠黏膜→腺瘤→腺癌的顺序呈递变趋势 ,伴有淋巴结转移的腺癌组中 ,上述各参数值明显增高。p5 3与PCNA呈正相关 ,与组织分化程度无关。结论 :定量检测p5 3、PCNA对判断大肠癌的恶性程度。
Objective To evaluate expression of p53 and PCNA in colorectal tumor and its clinopathological correlection andprognostic significance.Methods 10 normal colonic mucosae,30 colorectal adenomas and 53 colorectal carcinomas with complete follow up data were examined for PCNA and p53 with quantitative computed processed imaging analysis and SP immunohistochemical technique.Results p53 and PCNA positive rate were 52 38%,97 73%.The values of the parameters analyzed increased in accordance with the normal mucosa adenoma adenocarcinoma sequence,and were higher in patient with lymph node metastasis evidently p53 positive tumors always coexisted with over expression of PCNA.There was no significant correlation between p53 expression and differentiated degree of cancer.Conclusions p53 and PCNA may be useful in evaluating malignancy degree and prognosis.The quantitative evaluation of some indices of colorectal tumor can provide reproducible data for differential diagnosis of this tumor.
出处
《肿瘤防治杂志》
CAS
2001年第6期613-615,共3页
China Journal of Cancer Prevention and Treatment
基金
福建省教委科研基金资助 ( 97A0 6 8)
