摘要
目的 :探讨外周血T淋巴细胞核仁区嗜银蛋白 (Ag NoRs)测定对判断肿瘤细胞分化程度的意义。方法 :5 0例健康查体者 ,63例经手术后病理证实且经病理组织细胞学分型的恶性肿瘤患者。根据其病理分型 ,分为低分化组 ( 2 3例 )、中分化组 ( 2 1例 )和高分化组 ( 19例 )。取静脉血 0 5mL(肿瘤患者均在术前 3d取血 ) ,经培养、制片、银染、图象分析 4个步骤 ,以T淋巴细胞核仁银染面积与细胞核面积比值 (I S %值 )作为Ag NoRs的检测指标。结果 :健康组与恶性肿瘤组I S %均值分别为 7 2 3± 0 4 5、4 5 2± 0 2 5 ;二者比较差异有显著意义 ,P <0 0 5。在肿瘤患者中 ,低分化组、中分化组、高分化组 1 S %均值分别为 2 76± 0 13、4 82± 0 35、5 2 1± 0 2 3,经统计学分析 ,低分化组外周血T淋巴细胞Ag NoRs含量明显低于中、高分化组。结论 :外周血T淋巴细胞Ag NoRs测定不仅对诊断肿瘤具有重要意义 。
Objective To evaluate the significance of the analysis of Nucleolar Organizer Region Associated Argfrofil Proteins(Ag-NORs) in peripheral blood T-lymphatic cell for judging the differentiation degree of malignant tumor.Metheds Perpheral blood (0.5 mL) from 50 normal volunteers and 63 patients with malignant tumor in which there were low differentiation degree (LDD)23 cases,moderate differentiation degree(MDD) 21 cases and high differentiation degree(HDD)19 cases according to differentiation degree proved by pathology was cultured,smeared,sliver-dyed,analized,using I.S%(integral areas /nucleus integral areas)as the index of Ag-NORs.Results I.S% Mean was 7.23±0.45 in normal cases and 4.52±0.25 in patient with malignant tumor.A significant difference was found,P<0.05.In malignant tumor cases,the I.S% mean of LDD groups was 2.76±0.13,MDD groups 4.82±0.35 and HDD groups 5.21±0.23.The Ag-NORs in the LDD groups was significantly fewer than that in the MDD and HDD.There was a statistically difference,P<0.05.Conclusions The analysis of Ag-NORs in peripheral blood T lymphatic cell has significant value not only in the diagnosis of malignant tumor,but also in the judgement of malignant tumor's differentiation degree.
出处
《肿瘤防治杂志》
2003年第4期360-361,共2页
China Journal of Cancer Prevention and Treatment
关键词
肿瘤
病理学
T淋巴细胞
核蛋白类
核仁组成区
细胞分化
嗜银蛋白
测定
neoplasms/pathology
T-lymphocytes
nucleoproteins/analysis
nucleolar organizer region
case-control studies
