摘要
以几丁质为载体,戊二醛为交联剂,固定壳聚糖酶,对壳聚糖酶的固定化条件、固定化酶的性质进行了研究,确定了酶固定的最佳条件为0.1克几丁质与5ml5%戊二醛交联,固定2mg壳聚糖酶,在此条件下酶活力回收可达70%。固定化酶的最适温度和pH分别为60℃和4.0,动力学参数Km值为17.66g/L。将固定化酶于70℃水浴保温150min,酶活力未见明显下降。该固定化酶具有良好的操作和保存稳定性。
Chitosanase was immobilized on chitin with glutaraldehyde by cross-link reaction,the immobilization conditions and characterization of the immobilized enzyme were studied.The optimal conditions for immobilization were as follows:0.1g chitin was treated with 5ml 5% solution of glutaraldehyde,then 2mg chitosanase was immobilized on the carrier.Optimal temperature and pH for the immobilized enzyme were 60℃ and 4.0 respectively.Kinetic parameter Km was 17.66g/L.The immobilized enzyme showed good temperature stability,and its operation and storage stability were also very good.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第10期21-24,共4页
Food Science
基金
浙江省自然科学基金资助项目(批准号:200075)
