摘要
应用 RAPD方法对药用野生稻、普通野生稻、粳稻和籼稻进行基因组多态性分析。 1 2个随机引物共扩增出 1 3 2条 RAPD带 ,片段大小在 3 0 0～ 3 5 0 0 bp之间 ,其中有 1 0 6条表现出多态性 ,占总扩增片段的86.4%。根据遗传距离分析 ,用 UPGMA法构建了聚类树状图 ,结果表明 ,普通野生稻的遗传特性比药用野生稻更接近于栽培稻。
The genomic DNA variations of wild rice(Oryza officinalis and Oryza rufipogon)and cultivated rice were analyzed using random amplified polymorphic DNA(RAPD). Twelve arbitrary primers generated 132 RAPD bands with the size ranging from 300 bp to 3 500 bp, 106 of these bands were polymorphic(86.4%). The UPGMA cluster was conducted on the base of matrix of genetic distance. It was shown that O. rufipogon was closer to the cultivated rice than the O. officinalis.
出处
《广西植物》
CAS
CSCD
北大核心
2001年第4期339-343,共5页
Guihaia
基金
国家转基因植物研究与产业化专项资助 (编号 :J0 0 - A- 0 0 3 )
