摘要
目的揭示籼粳基因组的多态性。方法选用分布于水稻12条染色体上的RFLP对多个水稻品种进行分析,结合生物信息学方法分析这些籼粳特异性标记的籼粳特异性及其序列分子的生物学基础。结果筛选到28个RFLP籼粳特异性探针,籼粳特异性标记的生物信息学分析表明,这些标记在基因组水平上存在差异。结论这些标记能对水稻品种进行籼粳稻分子分类。水稻基因组序列图的完成和释放,使得这些籼粳特异性标记揭示的基因组多态性可以在基因组序列水平上进行分析和验证。
[Objective] Revealing the genome polymorphisms between Indica and Japonica subspecies. [Method] RFLP markers, which are distributed on 12 chromosome of rice, were used to analyze Indica-Japonica differentiation in different rice varieties. The same time, genome sequence variations of screened loci were analyzed by bioinformatics method. [Result] Twenty-eight RFLP probes, which can classify Indica-Japonica rice, were confirmed. Subspecies genome polymorphisms of screened loci were found by analyzing the publication of the genome sequences data of rice. [ Conclusion ] The study indicated that these screened markers can be used for classifying Indica-Japonica subspecies. With the publication of the genome sequences of rice, marker polymorphisms between Indica and Japonica subspecies can be revealed by genome sequence differentiation.
出处
《中国农业科学》
CAS
CSCD
北大核心
2006年第5期1038-1043,共6页
Scientia Agricultura Sinica
基金
国家水稻基因图谱研究项目(101-09-06)
天津市导向重大项目(973122711)
PRO-TAC国际合作项目资助(Ref:2004/2001)
天津市自然科学基金项目(01306611)
