摘要
本研究以57个中国大豆祖先品系及育成品种和18个美国大豆祖先品系为DNA样品来源,通过随机引物PCR扩增基因组DNA的多态性,探索利用RAPD标记鉴定相关种质的可能性。研究结果表明,50个10摩尔随机引物共扩增可分辨产物246个,其中82.4％的随机引物可产生多态性产物,所扩增产物的54.4％至少在两个基因型间存在差异。每个PCR扩增产物分别以1和0记录存在与否。扩增产物间的成对比较可产生非相似性矩阵,此矩阵用于构建遗传相似性的树状系谱图。聚类分析结果表明,中国大豆种质与美国大豆种质不同,两国内的南北方种质也不同。这种明显的地理差异为丰富中国大豆品种遗传基础的种质选择提供了理论依据。
Fifty-seven Chinese ancestral lines and their derived cultivars and eighteen US ancestral lines were used to evaluate genetic diversities by RAPD markers between or within Chinese and US lines. Fifty 10-mer random primers were used for PCR reactions. The total of 246 distinguishable PCR products were detected by agarose gel electrophoresis among 75 accessions, 82. 3% of random primers were polymorphic and 54. 4% of total produts were different at least between two lines, which can be used as RAPD markers to identify soybean germplasm resources. Thirty-two soybean accessions had their special PCR banding patterns. Each of those amplified bands was taken as one of the variant which was record as 1 for present and 0 for absent. Comparison among all possible a pair of data were calculated to produce non-similarity matrix and to build up a pedigree tree. The cluster analysis indicated that not only Chinese ancestral lines were different from US ancestral lines, but northern soybean accesstions were different from southern ones within two countries also. The obvious geographic differences provide possibilities to broaden genetic base of Chinese soybean cultivars by selecting germplasm from various regions within or between countries.
出处
《作物学报》
CAS
CSCD
北大核心
1997年第4期408-417,共10页
Acta Agronomica Sinica
基金
中美大豆品种资源合作研究项目
