摘要
目的： 研究日本血吸虫重组BCG-Sj26GST疫苗对小鼠脾细胞IL-6的影响。方法：实验1采用该疫苗皮下免疫小鼠，免疫后8周用日本血吸虫尾蚴进行攻击感染，感染后6周剖杀小鼠，同时设有PBS对照组。实验2用该疫苗皮下和静脉注射分别免疫小鼠，于免疫后0、4、8、10、14和16周各剖杀4只，分离脾脏，用Sj26或PHA刺激脾细胞，用ELISA法检测脾细胞上清液中IL-6含量。结果：疫苗免疫,尾蚴攻击后，IL-6水平无明显变化；动态观察发现IL-6于疫苗免疫后8～10周达最高水平。结论：IL-6可能与日本血吸虫重组BCG-Sj26GST疫苗诱导的保护性免疫力无关。
Objective: To study the effect of recombinant BCG-Sj26GST vaccine of schistosoma japonicum on IL-6 produced by spleen cells of mice. Methods: In the first experiment, mice were immunized subcutaneously by the vaccine, with PBS serving as control, challenged with Sj cercariae after 8w of immunization and killed on 6w of the infection to separate spleens; in the second experiment, after subcutaneous and intravenous immunization by the vaccine respectively, 4 mice were killed to separate spleens on 0,4,8,10,14 and 16w of immunization, spleen cells were stimulated by Sj26 or PHA, and the level of IL-6 in supernatant of spleen cells were detected by ELISA. Results: The level of IL-6 had no obvious change by immunization with the vaccine against challenge with Sj cercariae;dynamic observation showed that IL-6 reached the highest level on 8~10w of immunization. Conclusion: IL-6 may not be related to the protective immunity elicited in mice by vaccination with recombinant BCG-Sj26GST vaccine of schistosoma japonicum.
出处
《重庆医科大学学报》
CAS
CSCD
2001年第2期148-150,共3页
Journal of Chongqing Medical University
