摘要
目的研究肿瘤坏死因子α（ＴＮＦα）和表皮生长因子（ＥＧＦ）对人、牛晶状体上皮细胞增殖的影响。方法采用ＭＴＴ比色法检测ＴＮＦα和ＥＧＦ对晶状体上皮细胞（ｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌ，ＬＥＣ）增殖的作用。结果０．１Ｕ／ｍｌ的ＴＮＦα即可明显促进人、牛晶状体上皮细胞的增殖；ＥＧＦ为１、１０ｎｇ／ｍｌ时可明显促进牛晶状体上皮细胞增殖。结论细胞因子ＴＮＦα。
ObjectiveTo examine the effects of tumor necrosis factor α (TNF α)and epidermal growth factor(EGF)on the proliferation of human and bovine lens epithelial cells(LECs).MethodsHuman LECs obtained during cataract surgeries were cultured in DMEM with 20% fetal calf serum (FCS) and bovine LECs were cultured in DMEM with 10% FCS.During the experiments,LECs were seeded with different concentrations of TNF α or EGF.MTT assay was used to assess the effects of the cytokines on the proliferation of LECs.Results0.1～1000U/ml TNF α could significantly stimulate the proliferation of human and bovine LECs.When the concentration of EGF was 1 or 10 ng/ml,it could effectively stimulate the proliferation of bovine LECs.ConclusionThese findings suggest that TNF α and EGF participate in posterior capsular opacification by simulating the proliferation of LECs.
出处
《眼科研究》
CSCD
1998年第2期90-92,共3页
Chinese Ophthalmic Research
