摘要
目的研究肿瘤坏死因子α （ＴＮ－α）对体外培养的牛眼晶状体上皮细胞中原癌基因 ｆｏｓ，ｊｕｎ， ｍｙｂ， ｍｙｃ， ｒａｓ等蛋白产物表达的作用。方法细胞经 １０ Ｕ／ｍｌ ＴＮＦ－α作用不同时间后，采用卵白素－生物素－过氧化物酶复合物（ＡＢＣ）免疫酶标技术测定蛋白表达。结果经ＴＮＦ－α作用后，几种原癌基因表达的阳性细胞百分率均明显增高（Ｐ＜０．０１）。 ｆｏｓ，ｊｕｎ，ｍｙｂ，ｒａｓ在刺激１ｈ后即达到顶峰，ｍｙｃ在刺激２ｈ后达到顶峰，随后很快下降，恢复至正常水平。同时，细胞的阳性反应强度增加，细胞核内变化更为明显。结论ＴＮＦ－α通过激活细胞内原癌基因的表达而促进晶状体上皮细胞的增殖。
Objective To assess the effects of tumor necrosis factor α (TNF-α) on the expression of proto-oncogenes (fos,jun,myb, myc,ras) in bovine lens epithelial cells (LECs) in vitro. Methods The third passage LECs were used for the experiments. 5×10/ml cells were seeded in the presence of 10U/ml TNF-α using 24-well tissue culture plate. After 1,2,4,8h, the protein expression was assessed by avidin-biotin-peroxidase complex immunostaining method. Results The incubation with TNF-α increased the percentage of positive cells and the intensity of staining significantly(P<0.01). The expression of fos,jun, myb, ras proteins reached the highest level after incubation for 1 h, so did myc protein after 2 h-incubation. Jun and ras expression lasted shortly,returning to control level within 4 h, while that of fos and myb returned to control level until & h. All these changes were more apparent in nucleus. Conclusion TNF-α could stimulate proto-oncogene expression, prior to its effect on cell proliferation. TNF-α could stimulate expression of proto-oncogenes to regulate cell cycle. It may play an important role in posterior capsule opacification through promoting LECs proliferation.
出处
《眼科研究》
CSCD
2000年第4期336-338,共3页
Chinese Ophthalmic Research
