摘要
应用组织块静置培养法建立成年水牛晶状体上皮细胞的离体培养方法,通过细胞计数法、MTT法和透射电镜法观察,对晶状体上皮细胞的离体培养和冻存复苏的可行性进行研究,结果显示离体培养和冻存复苏后的晶状体上皮细胞生长良好且二者无显著差异(P>0.05),并保持了原有上皮细胞的特性及细胞结构。提示组织块静置培养法是适宜的晶状体上皮细胞培养方法,液氮冷冻可有效地保存晶状体上皮细胞。
The modified method for cultivating an adult buffalo lens epithelial cells was described. The cell growth in vitro and the recovery of cells from frozen stale was investigated by cell counting ?MTT and transmission electron microscopy. The results appeared that there is no significant difference in growth between cells cultured in vitro and recovered from frozen stale (P>0. 05), with characteristic and structure similar to the normal epithelial cell. The results suggested that planting culture is a kind of culture method for lens epithelial cells and freezing is an effective method to keep in cryopreser-vation such cells.
出处
《上海实验动物科学》
CAS
2002年第4期223-226,共4页
Shanghai Laboratory Animal Science
