摘要
目的 研究白内障患者晶状体囊膜中的成分。方法 采用间接免疫荧光法研究白内障术中取出的患者晶状体囊膜的成分。结果 Ⅳ型胶原、层粘蛋白阳性率为 10 0 % ,部分患者晶状体囊膜中有纤维连接蛋白、Ⅰ型胶原存在。有些囊膜同时具有上述 4种成分。结论 白内障患者的晶状体囊膜主要成分依然为Ⅳ型胶原和层粘蛋白 ,但也会有纤维连接蛋白、Ⅰ型胶原等成分存在。这些细胞外基质成分可能在白内障术后晶状体上皮细胞的移行、增殖中发挥作用。
ObjectivePosterior capsule opacification (PCO) is a common complication after cataract surgery.Several previous studies suggest that the residual lens epithelial cells (LECs) proliferation,migration and differentiation onto the posterior capsule lead to PCO.The aim of this study was to detect the extracellular matrix components in cataract lens capsule,in order to find the biological basis for LECs’ action.MethodsThe lens capsules obtained during cataract surgeries were pasted on the glasses coated with 3′ aminopropyl triethoxy silane(APES).After they were dried at room temperature,they were assayed by indirect immunofluorescence to detect the presence of type Ⅰ and Ⅳ collagen,laminin,fibronectin.The secondary antibody was labeled by FITC.ResultsAll the capsules examined were Ⅳ collagen and laminin positive.While only part of the capsules showed positive reactions with anti fibronectin antibody or anti type I collagen antibody.The positive percentages of fibronectin,type I collagen were 62 8% and 91 4%,respectively.It was noticeable that some capsules had the presence of all four extracellular matrix components.ConclusionCompared to the normal lens capsule,the main components of the cataract counterpart are still type Ⅳ collagen and laminin.But fibronectin and type I collagen are indeed present in some patients’ capsules.These extracellular matrixes may play a role in the migration and proliferation of lens epithelial cells after cataract surgery.
出处
《眼科研究》
CSCD
2000年第3期207-209,共3页
Chinese Ophthalmic Research
