摘要
目的研究白细胞介素１（ｉｎｔｅｒｌｅｕｋｉｎ１，ＩＬ－１）、肿瘤坏死因子α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒα，ＴＮＦ－α）对体外培养的小牛晶体上皮细胞（ｂｏｖｉｎｅｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌ，ＢＬＥＣ）增殖和胞浆内游离Ｃａ２＋浓度的影响，探讨它们在后囊混浊形成中的作用及其影响细胞增殖的机理。方法采用ＭＴＴ比色法测定ＩＬ－１及ＴＮＦ－α对细胞增殖的影响，用荧光指示剂Ｆｕｒａ－２测定它们对胞浆内游离Ｃａ２＋的影响。结果ＩＬ－１１０２～１０５ｎｇ／ｍｌ、ＴＮＦ－α１０２～１０４Ｕ／ｍｌ可显著促进细胞增殖，并增加胞浆内游离Ｃａ２＋浓度。结论推测ＩＬ－１、ＴＮＦ－α参与了白内障术后的后囊混浊形成；它们影响细胞增殖的作用机理之一是通过胞浆内游离Ｃａ２＋。
Objective To study the effects of interleukin 1（IL-1）and tumor necrosis factor α（TNF-α）on the proliferation and cytosolic Ca^2＋ of bovine tens epithelial cells（BLEC） in vitro. The effects of IL-1 and TNF-α on posterior capsular opacification （PCO） and the mechanism of effect on the proliferation were investigated. Methods The effects of IL-1 and TNF-α on the proliferation of BLEC were detected with MTT staining colorimetry, and cytosotic Ca^2＋ was measured by fluoresence determination with Fura-2. Results IL-10^2- 10^5 ng/ml, TNF-α 10^2- 10^4 U/ml obviously could promote BLEC proliferation（ P〈0. 05 - 0.01 ）, and increase cytosolic Ca^2＋ （ P 〈 0. 05- 0. 01 ） at theTNF-α on proliferation is by cytosolic Ca^2＋.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1998年第2期106-108,共3页
Chinese Journal of Ophthalmology
