摘要
目的]探讨IL_18及乙肝病毒S基因(HBVS)的联合功效。[方法]从胎肝组织中抽提总RNA ,RT-PCR扩增IL-18cDNA基因。从载体 pcDNA3.0/S中限制性酶切获取HBVS基因 ,然后 ,将其与IL -18cDNA一并亚克隆到真核表达质粒 pIRES-EGFP中。[结果]构建了具有抗肿瘤及乙肝双重功效的IL18_HBVS乙肝基因疫苗。
To find the combination function of IL_18 and HBV S gene. IL_18 cDNA was obtained by RT_PCR from human embryo liver tissue. And an eukaryote expression vector _pEGFP_N1/IL_18 with a report gene which was expressing green fluorescene protein was constructed. Then the authors got the IL_18 cDNA by restriction enzyme and PCR.Because of its liverphagy and immunization ,the authors used HBV S gene from an expression vector_pcDNA3.0/S and linked it with IL_18 cDNA . This two genes were subcloned into an expression eukaryote vector_pIRES_EGFP and formed a new expression vector pIRES_EGFP_S_IL18 that would resist tumor and Hepatitis B.[Conclusion]The recombination of IL18_HBV S gene makes great progress in enhancing the immunity of HBV gene vaccine.It could play a basic role in resisted tumor and Hepatitis B.
出处
《中国肿瘤》
CAS
2001年第4期233-235,共3页
China Cancer
基金
国家自然基金课题! (39970346)
