摘要
目的 探讨人工合成的含CpG基序的寡核苷酸 (ODN)作为佐剂对乙型肝炎病毒(HBV)基因疫苗诱导小鼠产生细胞免疫应答的影响。方法 构建编码HBV表面抗原蛋白S的重组真核表达质粒 pCR3.1 S作为HBV基因疫苗 ,人工合成含CpG基序 (motif)的硫代磷酸寡核苷酸作为佐剂 ,以Balb/c小鼠作为实验动物进行免疫接种 ;采用3 H TdR法、51Cr 4h释放法等分别检测免疫小鼠的淋巴细胞增殖和杀伤功能。结果 与空载体对照组相比较 ,HBV基因疫苗诱发Balb/c小鼠产生较好的HBV特异的淋巴细胞增殖及杀伤效应 (P <0 .0 5 ) ;应用CpGODN组与未应用组相比较 ,免疫小鼠产生更强的HBV特异性细胞免疫应答 (P <0 .0 5 )。
Objective To investigate the effects of CpG oligodeoxyribonucleotide (ODN) as adjuvants on cellular immune responses in mice immunized with hepatitis B virus (HBV) gene vaccines. Methods Recombinant eukaryotic expression plasmids of pCR3.1 S encoding S protein of HBV were constructed as gene vaccines. Phosphorothioate oligodeoxynucleotides containing one CpG motif were synthesized as adjuvants. Then Balb/c mice were inoculated so that the immune responses to HBV could be studied. Lymphocyte proliferation reactions and cytotoxic T lymphocyte activities were detected through 3H TdR incorporation and 4 hour 51 Cr release assays. Results Compared with plasmid vectors of pCR3.1, HBV gene vaccines could induce strong lymphocyte proliferation reactions and cytotoxic T lymphocyte activities in immunized mice ( P <0.05). Immunized mice with CpG ODN showed higher specific cellular responses to HBV than those without CpG ODN ( P <0.05). Conclusion CpG ODN can act as adjuvants to enhence cellular immune responses in mice immunized with HBV gene vaccines.
出处
《中华传染病杂志》
CSCD
北大核心
2000年第2期80-83,共4页
Chinese Journal of Infectious Diseases
基金
国家自然科学基金! ( 3 9770 665 )
关键词
乙型肝炎
HBV
CPG基序
ODN
基因疫苗
免疫应答
CpG oligodeoxyribonucleotide
Hepatitis B virus
Gene vaccine
Cellular immunity response
