摘要
目的构建乙肝病毒（ＨＢＶ）基因疫苗，观察其与ＨＢＶ表面抗原蛋白（ＨＢｓＡｇ）联合免疫小鼠诱导的免疫应答．方法构建重组真核表达质粒ｐＣＲ３１Ｓ作为ＨＢＶ基因疫苗，联合应用纯ＨＢｓＡｇ蛋白免疫Ｂａｌｂ／ｃ小鼠，以单用基因疫苗ｐＣＲ３１Ｓ或纯蛋白ＨＢｓＡｇ免疫小鼠作为对照组．采用ＥＬＩＳＡ法检测免疫小鼠血清抗ＨＢｓ，另取免疫小鼠脾细胞，用３ＨＴｄＲ掺入法测定各组免疫小鼠的淋巴细胞增殖活性．结果２，４ｗｋ时联合免疫组抗ＨＢｓ效价低于纯蛋白免疫组，高于基因疫苗免疫组；６ｗｋ后基因疫苗免疫组抗ＨＢｓ效价升至最高，联合免疫组次之．３ＨＴｄＲ掺入法测定显示，各组免疫小鼠的淋巴细胞增殖反应差异不显著．结论乙肝病毒基因疫苗与表面抗原蛋白联合免疫无优势．
AIM To investigate the immune responses in mice immunized with gene vaccines of
hepatitis B virus (HBV) in combination with hepatitis B surface protein (HBsAg). METHODS
Eukaryotic expression plasmids of pCR3 1 S were constructed as HBV gene vaccines. Balb/c
mice were immunized with HBV gene vaccines plus HBsAg protein, and control groups of mice
were immunized with HBV gene vaccines or HBsAg protein. Anti HBs antibodies in vaccinated
mice were measured by ELISA, and lymphocyte proliferation activity of immunized mice was
detected through 3 H-TdR incorporation. RESULTS At the 2nd and 4th week, mice in
combination group had lower anti HBs titer titers than mice in HBsAg protein group and higher
anti HBs titer than mice in gene vaccines group. But at the sixth week, mice in gene vaccines
group had the highest anti HBs titers and mice in combination group had the second highest
titers. In addition, it appeared that lymphocyte proliferation activity among each group had no
significant differences detected through 3 H TdR incorporation. CONCLUSION
Immunization with HBV gene vaccines plus HBsAg protein produces no better immune
responses.
出处
《世界华人消化杂志》
CAS
1999年第3期188-190,共3页
World Chinese Journal of Digestology
基金
国家自然科学基金
