摘要
:目的 :观察灵芝多糖体外对小鼠T细胞蛋白激酶A (PKA)和蛋白激酶C(PKC)活性是否有影响。方法 :采用反相离子对高效液相色谱法测定PKA和PKC活力。结果 :灵芝多糖GLB7 能引起T细胞中PKA和PKC活性明显增强并具有剂量依赖性 ,达峰时间分别为5min和20min ,于20min及1h分别恢复到基础水平 ;GLB7 还可引起T细胞中PKC发生质膜转位 ,并拮抗Stau rosporine对T细胞中PKC的抑制作用。结果
AIM:To investigated the effects of Ganoderma polysaccthride(GLB7)on protein kinase A(PKA) and protein kinase C(PKC) activitives in murine T cells.METHODS:A new ion-pair reversed-phase high liquid chromatography method was used to determinate the activities of PKA and PKC in T cells.RESULTS:GLB7 could markedly increase the activities of PKA and PKC in murine T cells in a dose-dependent manner.The peak time was at 5 min and 20 min and the activities of PKA and PKC returned to basic level at 20 min and 1.5h respectively.GLB7 could induce translocation of PKC and antagonize the inhibition effect of staurosporine(10μmol·L-1) on PKC in T cells.CONCLUSION:The immunopotentiating and antitumour effects of Ganoderma polysacchride may be associated with its activation on PKA and PKC in murine T cells.
出处
《中国药房》
CAS
CSCD
2001年第2期78-79,共2页
China Pharmacy
基金
国家自然科学基金资助项目! (39400165)
