摘要
目的:研究灵芝多糖(GLB7) 对小鼠巨噬细胞(MФ) 内cAMP含量的影响,为其免疫增强作用机制提供依据。方法:采用细胞培养和竞争性蛋白结合分析法测定小鼠腹腔MФ中cAMP含量。结果:GLB7 能剂量依赖性引起小鼠腹腔MФ中cAMP浓度快速升高,5 min 达峰值,之后缓慢下降,至30 min 时基本恢复原来水平。结论:GLB7 引起小鼠MФcAMP浓度快速升高,可能与其增强小鼠免疫功能有关。
Objective:Investigating the effect of GLB 7 on cAMP in murine peritoneal macrophages to provide a scientific evidence for the immunomodulatory mechanism.Method:Cell culture and radio immunological assay of cAMP were used.Result:GLB 7 increased the production of cAMP in a concentration and time dependent manner in murine peritoneal macrophages.Conclusion:The immunopotentiating effect of GLB 7 may be due to the activation of macrophages that leads to the increase of cAMP.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2000年第1期41-43,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金!(39400165)