摘要
以疏花蔷薇(Rosa laxa,2n=2x=14)和现代月季品种‘Kardinal’、‘Solidor’、‘Yuzen’(2n=4x=28)为亲本进行杂交,杂交组合疏花蔷薇בKardinal’、疏花蔷薇בSolidor’、‘Yuzen’×疏花蔷薇分别获得5株、5株和6株杂交后代。采用常规压片法,对亲本及杂交后代的染色体数目和核型进行了分析。结果显示:杂交后代中除疏花蔷薇בKardinal’组合有1株为二倍体外,其余15株均为三倍体;疏花蔷薇的核型为12m+2sm,属1A型;现代月季品种‘Kardinal’、‘Solidor’、‘Yuzen’的核型分别为28m、28m、24m(2SAT)+4sm,核型类型分别为1A、1B、2B型;疏花蔷薇בKardinal’组合的F1代包括1A、1B和2A等3种核型;疏花蔷薇בSolidor’组合的F1代包括1A和1B等2种核型;‘Yuzen’×疏花蔷薇组合的F1代包括1A、1B和2B等3种核型。根据染色体数目和形态分析结果确定,获得的三倍体后代为真杂种,但对于二倍体后代的杂种真实性不能做出判断。
In this study, hybridization was conducted between Rosa laxa （2n= 2x= 14） and Rosa cv. ‘ Kardi- nal＇, ‘Solidor＇ ,‘ Yuzen＇ （2n= 2x= 28）, and five, five and six F1 hybrids were obtained from cross combina- tions of R. laxa × ＇Kardinal＇ ,R. laxa × ＇ Solidor＇ and R. laxa × ＇Yuzen＇, respectively. The karyotypes of parents and their interspecific hybrids were analyzed using traditional squashing method. The results showed that 15 F1 hybrids were triploid, except that one of the hybrids between R. laxa and ‘Kardinal’ was diploid. The karyotype formula of R. laxa was 12m＋2sm belonging to 1A. Karyotype formulas of ＇Kardinal＇, ＇Solidor＇, ＇Yuzen＇ were 28m,28m,24m（2SAT）＋4sm,respectively,of which belonged to 1A, 1B and 2B. Karyotype formulas of hybrids of R. laxa× ＇Kardinal＇ included 1A,1B and 2A. Karyotype for- mulas of hybrids of R. laxa× ＇Solidor＇ included 1A and lB. Karyotype formulas of hybrids of ＇Yuzen＇ ×R. laxa included 1A, 1B and 2B. According to the karyotype characters,triploid F1 hybrids were true proge- ny,however,the hybrid authenticity identification of diploid F1 hybrids could not be confirmed.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第3期488-494,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
十二五科技支撑计划(2012BAD01B07
2013BAD01B07)
北京市共建项目专项
关键词
月季
疏花蔷薇
杂交
倍性
核型分析
rose
Rosa laxa
hybridization
ploidy level
karyotype analysis
