摘要
建立了测定烟用香精香料中18种氨基酸的液相色谱-串联质谱（LC-MS/MS）方法.以L-正缬氨酸（Nva）为内标,样品经10％甲醇-水溶液（盐酸浓度为0.1 mol/L）萃取、0.22 μm水相滤膜过滤后直接进行LC-MS/MS分析.结果表明：18种氨基酸在0.1～10 μmol/L范围内,线性相关系数均≥0.9990,3个加标水平的回收率在85.4％～110.0％之间,相对标准偏差（RSD）为1.3％～4.9％.方法的检出限（LOD）和定量限（LOQ）分别为0.00005～0.1和0.0002～0.3 μmol/L.该方法准确、灵敏,适合用于烟用香精香料样品中氨基酸的测定.
A method for determining 18 amino acids in tobacco flavors by liquid chromatography tandem mass spectrometry （LC-MS/MS） was developed. Using L-norvaline （Nva） as an internal standard, the sample was extracted by 10% methanOl-water solution （HC1 concentration of 0.1 mol/L）, and the extract was directly analyzed by LC-MS/MS afte＇r filtering with a 0.22 pm aqueous phase filtration membrane. The results showed that： the linear correlation coefficients of 18 amino acids were above 0.9990 in the range of 0.1-10 μmol/L, and the recoveries of 3 standard addition levels ranged from 85.4% to 110.0% with the relative standard deviation （RSD） of 1.3%-4.9%. The limits of detection （LODs） and the limits of quantification （LOQs） of the method were 0.00005-0.1 μmol/L （S/N=3） and 0.0002-0.3 μmol/L （S/N=10）, respectively. This method is accurate, sensitive and suitable for determining the amino acids in tobacco flavors.
出处
《烟草科技》
EI
CAS
北大核心
2014年第3期56-59,70,共5页
Tobacco Science & Technology
基金
国家烟草专卖局卷烟减害技术重大专项子项目"七匹狼卷烟主流烟气7种成分释放量影响因素研究"(110200902013)
