摘要
采用离子色谱-积分脉冲安培法同时测定了烟草中18种游离氨基酸的含量。结果表明,该方法同时检测烟草中18种游离氨基酸的平均回收率为80%～115%,最低检出限为0.03～0.75μg/mL,线性相关系数为0.9900～0.9992。该方法成功对比分析测定了烤烟、白肋烟和香料烟3种不同类型单料烟以及国内外不同风格卷烟中18种游离氨基酸的含量。
18 of free amino acids in tobacco were simultaneously determined by high performance anion-exchange chromatography with integrated pulsed amperometric detection method. The results showed that the recoveries for 18 amino acids were in the range of 80%-115 %, the detection limits for 18 amino acids were 0.03-0.75 ~tg/mL and the calibration graphs of peak area for the analyses were linear over about three orders of magnitude with a correlation coefficient of 0.9900-0.9992. The method was applied to determine 18 free amino acids in tobacco with satisfactory results. The method was employed to determine 18 free amino acids in flue-cured tobacco, burley tobacco and oriental tobacco. The method was also employed to determine successfully 18 free amino acids of different brands of domestic cigarettes and overseas cigarettes.
出处
《中国烟草科学》
CSCD
2012年第4期51-54,共4页
Chinese Tobacco Science
基金
山东中烟工业公司资助项目(110000038)
关键词
离子色谱
积分脉冲安培法
游离氨基酸
卷烟
ion chromatography with integrated pulsed amperometric detection method
free amino acid
cigarette
