摘要
目的 :研究 HSP70复合物抗人肝癌细胞的免疫效应。方法 :用经 42℃热休克处理的 SMMC- 772 1细胞制备裂解液 ,免疫共沉淀法提取 HPS70复合物 ,采用 SDS- PAGE和 Western- blot鉴定蛋白性质及分子量 ;蛋白测定仪进行复合物定量。 MTT法检测 HSP70复合物体外致敏人脾淋巴细胞对 SMMC- 772 1的细胞毒作用。结果 :SDS- PAGE和 Western- blot证实获得相对分子量约为 7× 10 4的 HSP70蛋白 ;体外细胞毒实施显示在不同效靶比时 ,致敏的淋巴细胞对 SMMC- 772 1均具有一定的杀伤效应 ,且这种效应可被 CD4、CD8的单抗部分阻断。结论 :HSP70复合物能在体外诱导针对人肝癌细胞的淋巴细胞毒效应 ,CD4、CD8介导了部分抗瘤作用 ,提示 HSP70可能通过多种免疫途径参与抗瘤效应。
Objective:To study the immunity effecf of HSP70 complex on human hepatocarcinoma cell line SMMC 7721.Methods:Cytosolic HSP70 complex of heat treated SMMC 7721 was obtained by immunoprecipitation.The molecular weight and the property of the HSP70 were identified by SDS PAGE and Western blot.The quantity of HSP70 complex was confirmed by protein detcting instrument.The cytotoxicity of human splenic lymphocytes to SMMC 7721 was examined through MTT.Results:HSP70 complex was obtained and further verified by Western blot.Ex vivo MTT test revealed though at differenet E:T ratios,splenic lymphocytes expressed a definite cytotoxicity effect on SMMC 7721 cell,which could be partly blocked by MAb anti CD 4 and anti CD 8.Conclusion:HSP70 complex could induce lymphocytes cytotoxicity effect on human hepatoma SMMC 7721 which partly mediated by CD 4 and CD 8.The research results will lay a better basis for further study on hepatoma vaccine and biotherapy.
出处
《肿瘤研究与临床》
CAS
2000年第6期367-369,共3页
Cancer Research and Clinic
