摘要
目的 应用联合修饰的树突状细胞(DC)在体外诱导高效而特异的抗胃癌免疫效应。方法 先将全长野生型p53导入脂质体内并转染小鼠骨髓来源的DC,然后用胃癌抗原肽-HSP70复合物等因素修饰已转染全长野生型 p53的 DC(wt-p53 DC),检测这种DC诱导小鼠脾脏淋巴细胞特异的细胞毒性T淋巴细胞(CTLs)的能力,分泌细胞因子功能,以及表面分子表达的高低。结果Western blot检测转染小鼠 p53cDNA的 BMDC及其培养上清中均可以检测到 p53表达;细胞因子含量明显增加(P<0.05),而的其他组的细胞因子含量与对照相比,无显著变化;经流式细胞仪(FACS)检测 wt-p53DC表面高表达 B7-1、B7-2、MCH-Ⅰ、MCH-Ⅱ;脾淋巴细胞经刺激后,能够特异性地杀伤胃癌细胞,杀伤率为91.6％。结论 全长野生型p53基因转染+抗原肽联合修饰DC能诱导小鼠细胞毒性T淋巴细胞的特异性,显著提高DC的抗原提呈功能。
Objective To induce efficient and specific anti--tumor immunological response in vitro
of dentritic cells transfected with full length wild type p53 and modified by GC-HSP70. Methods The
wild type p53 was transduced into BMDCs with liposome, and the DCs were modified by GC-HSP70. In-
duction of T lymphocyte to proliterate and differentiate to cytotoxic T lymphocyte (CTL) by wt-p53DCs
was detected. Results p53 expression was detectable in BMDC and the supernatant of the mice trans-
fected with p53cDNA. The contents of cytokines IL-12, TNF-α, IL-1β was increased in wt-p53DC modi-
fied by GC-HSP70 and GC (P < 0. 05 ). Flow cytometry revealed that B7--1, B7-2, MCH--Ⅰ, MCH-Ⅱ
were highly expressed on the surface of wt--p53DC. The T lymphocytes after stimulation could specifically
kill gastric cancer cells with the killing rate being 91. 6%. Conclusion The dendritic cells transfected
with wild type p53 and modified by GC-HSP70 can induce the specificity of mouse cytotoxic T lympho-
cytes and significantly increase the antigen presenting function of DC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第8期730-732,共3页
Chinese Journal of Experimental Surgery
