摘要
目的 对正常妊娠妇女及妊娠高血压综合征 (妊高征 )患者血管紧张素Ⅱ 1型受体(AT1R)基因A1166位点的多态性进行分析 ,探讨AT1R基因A1166位点的多态性与妊高征发病的关系。方法 采用聚合酶链反应 (PCR)、限制性酶切及电泳分型等方法 ,对正常妊娠妇女、妊高征患者及原发性高血压患者 (女性 )AT 1R基因A1166位点的多态性进行分析。结果 (1)正常妊娠妇女、妊高征患者及原发性高血压患者中AT 1R基因A1166位点的基因型频率 ,符合Hardy Weinberg平衡定律。 (2 )正常妊娠妇女 ,妊高征患者及原发性高血压患者中 ,AT 1R基因A1166位点C基因频率分别为 3.7% ,11.4%及 9.4%。 (3)妊高征患者中A1166→C变异体 (AC ,CC)出现率为 2 0 .5 % ,明显高于正常妊娠妇女的 7.4%。 (4)原发性高血压患者AT 1R基因A1166位点的变异基因型 (AC ,CC) ,和C等位基因的频率分别为 18.8%及 9.4% ,明显高于正常妊娠妇女 ;妊高征患者与原发性高血压患者的多态位点A1166→C基因型间差异无显著性 (P >0 .0 5 )。结论 (1)AT 1R基因A1166位点的变异 (A→C)的出现 ,可能增大了患妊高征的风险 ,并与妊高征发病有关。 (2 )妊高征患者可能携带了患原发性高血压的危险因素。
Objective To determine the distribution of gene type of A 1166 polymorphism site of the angiotensin Ⅱ type 1 receptor gene(AT1RG) and whether it might be implicated in pregnancy induced hypertension (PIH) woman. Methods We used polymerase chain reaction (PCR), restriction enzyme analysis and electrophoresis for this study. Results 1. The gene types of A 1166 polymorphism site of AT1R gene on normal control and PIH and essential hypertension subjects were in accordance with Hardy Weinberg laws. 2.The C allele frequencies of AT1R gene (A 1166 →C) in control, PIH, and essential hypertension subjects was 3.7%, 11.4%, and 9.4%, respectively. 3. The frequency of variants(AC,CC) of AT1R gene A 1166 polymorphism site in PIH (20.5%) was significantly higher than that of control subjects (7.4%). 4. The gene type of variants (AC,CC) and C allele frequency of AT1R gene A 1166 polymorphism site in essential hypertension (18.8%, 9.4%, respectively) was higher than those of control subjects. There is no statistical difference in A 1166 →C variants between PIH and essential hypertension. Conclusions 1.The variants(A→C) of 1166 polymorphsim site of AT1RG predisposes increased risk of PIH. 2.The PIH patients are at the risk of suffering from essential hypertension.
出处
《中华妇产科杂志》
CSCD
北大核心
2000年第3期136-138,共3页
Chinese Journal of Obstetrics and Gynecology
