摘要
目的观察小针刀疗法对肌张力增高大鼠背根神经节内PN3和NaN表达的影响。方法24只sD大鼠随机分为空白组、对照组和针刀组，采用类似股薄肌肌张力增高方法进行造模。对大鼠0～14天的机械痛阚进行连续测定，间断测量热痛阈。采用荧光定量PCR法对大鼠背根神经节PN3和NaN基因mRNA转录水平进行对比分析。结果针刀组第9天以后的机械痛阈与对照组比较，差异有统计学意义（P〈0．01），小针刀疗法明显改善了肌张力增高大鼠的机械痛阈。与对照组比较，针刀组第10天热痛阈改善明显（P〈0．05）。与空白组比较，对照组PN3mRNA明显上调（P〈0．01），小针刀针刺之后，PN3mRNA上调趋势得到阻止（P〈0．05）。结论小针刀疗法镇痛的分子通路与阻止电压门控钠通道PN3mRNA的上调相关。
Objective To observe impacts of small acupotomy on PN3 and NaN in dorsal root ganglion in rats with hypermyotonia. Methods Twenty-four Sprague-Dawley rats were randomly divided into a blank group, a control group and an acupotomy group. The method akin togracilishyperrnyotonia method was applied to establish model. The rats＇mechanical withdrawal thresholds were continuously measured from Day 1 to Day 14 and heat withdrawal thresholds were discontinuously measured. The fluorescent quantitation PCR method was used to compare and analyze mRNA transcript of PN3 and NaN gene in rats＇dorsal root ganglion. Results Compared with control group, mechanical withdrawal thresholds in the aeupotomy group since 9th day hadstatistical significance （P〈0.01）, indicating small acupotomy could obviously improve mechanical withdrawal threshold of hypermyotonia rats. Compared with control group, heat withdrawal thresholdsin the aeupotomy group onlOth days were significantly improved （P〈0.05）. Compared with the blank group, mRNA of PN3 in the control group was obviously increased （P〈0.01）. After treatment of small acupotomy, rising trend of mRNA of PN3 was halted. Conclusion The molecular pathway of small aeupotomy on analgesia is related with preventingthe rising of voltage-gated sodium channelPN3 mRNA.
出处
《北京中医药》
2013年第11期865-867,共3页
Beijing Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81001590)
关键词
小针刀
肌张力
背根神经节
PN3
NaN
small acupotomy
muscular tension
dorsal mot ganglion
PN3
NaN
