摘要
目的:研究马尾松等5种中药的乙醇提取物的体外抗氧化活性。方法:采用乙醇超声提取法获得各中药提取物,并以抗坏血酸(VitC)为阳性对照来考察其对超氧阴离子(O-2·)、羟自由基(·OH)、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)的清除效果。结果:甘草、丹参、马尾松、当归、罗汉松对O-2·的抑制率分别为(浓度相当于原药材100 g·L-1):15.86%,11.68%,11.31%,7.88%,5.94%;在清除DPPH·的实验中各中药的IC50(g·L-1)分别为:丹参1.6,马尾松1.87,罗汉松3.11,甘草13.91,当归22.32;而在清除·OH的实验中各中药的IC50(g·L-1)则为:丹参1.6,罗汉松2.02,马尾松2.1,甘草2.77,当归4.03;而三大体系阳性对照的IC50分别为0.047,0.93,0.21 g·L-1。结论:5种中药乙醇超声提取物均具有一定的抗氧化活性,其中丹参、马尾松效果较好;但与阳性对照Vit C相比仍有一定差距。
Objective：To study the preliminary antioxidant activities of ethanolextracts from several Chinese herbal medicine.Method：The in vitro scavenging activities of ethanol extracts by ultrasound on superoxide radical,hydroxyl radical and DPPH · were investigated with vitamin C （VC） as the positive control in this study.Result：The extracts of Glycyrrhiza uralensis,Salvia miltiorrhiza,Pinus massoniana,Angelica sinensis,Podocarpus macrophyllus had scavenging activities on superoxide radical （equivalent of raw material 100 g ·L-1,the eliminate rate was 15.86%,11.68%,11.31%,7.88%,5.94%,respectively）,DPPH ·（IC50value was 13.91,1.6,1.87,22.32,3.11 g·L-1,respectively）,·OH（IC50value was2.77,1.6,2.1,4.03,2.02 g·L-1,respectively）.The VC IC50 value of the three experiments was 0.047,0.93,0.21 g ·L-1.Conclusion：Five kinds of ethanol ultrasonic extract from Chinese herbal medicine have antioxidant activity,especially S.miltiorrhiza and P.massoniana,which hays the value of exploiting and utilizing.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期218-221,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科技厅重点项目(2011A030100010)
关键词
马尾松
丹参
抗氧化
自由基
体外
Pinus massoniana
Salvia miltiorrhiza
antioxidant activity
radical
in vitro
