摘要
目的研究天冬采收加工废弃物天冬皮的有效成分,为天冬加工过程中产生的废弃物的综合再利用提供参考。方法以天冬皮为原料超声提取总皂苷,经单因素与正交试验优化提取工艺,采用1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和2,2′-联氮-双(3-乙基苯并噻唑-6-磺酸)二铵盐阳离子自由基(ABTS~+·)探究体外抗氧化活性,并以秀丽隐杆线虫为模型,测定其对线虫寿命、运动能力、脂褐素、氧化与热应激的影响以评价抗氧化作用。结果最佳提取工艺为甲醇体积分数60%、温度50℃、料液比1∶30(g·mL^(-1))、超声3次,且每次30 min,此条件下,天冬皮总皂苷(total saponins of Asparagus Cochinchinensis peel,TSAC-P)含量平均值为0.46%,相对标准偏差(RSD)为0.104%。体外实验中,TSAC-P可降低自由基含量;秀丽线虫抗衰老研究中,TSAC-P能延长线虫自然寿命也能延长线虫在氧化应激和热应激胁迫下的寿命;TSAC-P能降低线虫脂褐素含量,提高线虫身体摆动频率。结论TSAC-P提取物具有较好抗氧化作用,可延长秀丽线虫寿命,促进线虫健康衰老。两种来源TSAC-P对线虫寿命的影响差异不明显,在0.8 mg·mL^(-1)质量浓度下效果最好。本研究首次揭示内江产天冬皮废料中皂苷组分的抗衰老活性,为其在功能食品添加剂和医药原料开发提供理论依据,同时在推动天冬资源“全株利用-循环经济”模式构建中起到积极作用。
OBJECTIVE To study the effective components of Asparagus cochinchinensis peel,a waste product of Asparagus cochinchinensis harvesting and processing,and to provide a reference for the comprehensive reuse of the waste produced in the processing of Asparagus cochinchinensis.METHODS Total saponins were extracted ultrasonically from Asparagus cochinchinensis peel,the extraction process was optimized by one-way and orthogonal tests,and the in vitro antioxidant activity was explored by DPPH free radical and ABTS+·free radical methods,and the effects on nematode lifespan,locomotor ability,lipofuscin,oxidative and thermal stress were determined to evaluate the antioxidant effects using the Hidradenitis occidentalis nematode as a model.RESULTS The optimal extraction process involves a methanol volume fraction 60%,a temperature of 50℃,a material-to-liquid ratio of 1:30(g·mL^(-1)),and ultrasound treatment conducted three times,each lasting 30 minutes.Under these conditions,the average total saponin content of Asparagus cochinchinensis peel(TSAC-P)is O.46%,with a relative standard deviation(RSD)of 0.104%.In vitro experiments demonstrate that TSAC-P can reduce free radical content.In studies on aging in Caenorhabditis elegans,these saponins are capable of extending the natural lifespan of the nematodes and also prolonging their lifespan under oxidative and thermal stress conditions.Additionally,TSAC-P can decrease the lipofuscin content in nematodes and increase their body movement frequency.CONCLUSION TSAC-P extract exhibits significant antioxidant properties,which can extend the lifespan of Caenorhabditis elegans and promote healthy aging in these nematodes.The impact of TSAC-P from two different sources on the lifespan of the nematodes showed no significant difference,with the optimal effect observed at a concentration of O.8 mg·mL^(-1).This study is the first to reveal the anti-aging activity of saponin components found in Asparagus peel waste from Neijiang,providing a theoretical basis for their development as functional food additives and pharmaceutical raw materials.Additionally,it plays a positive role in advancing the construction of a"whole plant utilization-circular economy"model for Asparagus resources.
作者
唐学博
张春
王刘艳
梁思雨
欧丽兰
TANG Xuebo;ZHANG Chun;WANG Liuyan;LIANG Siyu;OU Lilan(School of Pharmacy,Southwest Medical University,Luzhou 646000,China;Neijiang Dongxing District Traditional Chinese Medicine Health Industry Promotion Center,Neijiang 641000,China;Key Laboratory of Luzhou City for Aging Medicine,School of Pharmacy,Southwest Medical University Luzhou 646000,China)
出处
《中国药学杂志》
北大核心
2025年第21期2293-2302,共10页
Chinese Pharmaceutical Journal
基金
四川省科技厅应用基础计划项目资助(2021YJ0114)
泸州市科技计划项目资助(2023JYJ051)。
关键词
天冬皮
总皂苷
秀丽隐杆线虫
抗氧化
抗衰老
Asparagus cochinchinensis peel
total saponin
Caenorhabditis elegans
antioxidant
anti-aging
