摘要
采用顶空气相色谱法测定大豆磷脂中的丙酮残留量。色谱柱为 2m× 3mmi d 内填GDX 10 3的不锈钢柱 ,柱温为 16 0℃。试验结果的相对标准偏差为 1 2 % ,在 2 5 0 μg/g～ 10 0 μg/g范围内 ,回收率为 98 4%～10 4%。
Soybean phospholipids have many functions and alimentary actions In our country, powder soybean phospholipids are generally got by extraction with acetone,followed by vacuum drying There may be some residual acetone present in the soybean phospholipids, which is harmful to health So, we must know residual acetone content in the soybean phospholipids However we have not found a method to determine the residual acetone in the soybean phospholipids In this paper, headspace GC was used to determine residual acetone in powder soybean phospholipids The headspace bottle was glass with a volume of 15 milliliters Certain amounts of water, ammonium sulfate, and sample were added into the bottle. The mixture was made into a brei as soon as possible The bottle was put into a water bath at 40 ℃ for an hour The GC column was a 2 m×3 mm i d stainless steel tube packed with GDX 103 stationary phase Temperatures of both injector and detector were kept at 120 ℃ Column temperature was 160 ℃ Injection volume was 1 mL External standard method was used for quantitation The RSD was 1 2% The recoveries in the range of 25 0 μg/g 100 μg/g were 98 4% 104%.
出处
《色谱》
CAS
CSCD
北大核心
2000年第6期571-573,共3页
Chinese Journal of Chromatography
