摘要
目的:研究微波辐射对孕鼠脾淋巴细胞中活性氧(ROS)活性和线粒体膜电位(△Ψm)的影响。方法:32只成年雌性Wistar孕鼠随机分为4组,每组8只,其中1组为对照组,3组为实验组;实验组分别采用辐射强度为2.5、5、10 mW/cm2,辐射时间6 min/次,5次/每周,连续辐射4周,对照组不辐射;采用流式细胞术,间接检测细胞内ROS活性和△Ψm。结果:辐射强度为2.5 mW/cm2组细胞内ROS活性和△Ψm与对照组差异无统计学意义(P>0.05);辐射强度5、10mW/cm2组的ROS活性较对照组增加,△Ψm较对照组降低,差异有统计学意义(P<0.05),且呈剂量-反应关系。结论:长期低剂量微波辐射能够诱导孕鼠脾淋巴细胞中ROS活性增加,而△Ψm降低,并且具有一定的剂量效应规律性。
Objective： To research the effect of microwave radiation on reactive oxygen species （ROS） activity and mitochondrion membrane potential （ △ψm） of splenic lymphocytes in pregnant rats. Methods： Thirty - two adult male Wistar pregnant rats were randomly divided into one control group and three experimental groups, eight rats in each group; the rats in experimental groups were radiated by mi- crowave respectively （2.5, 5, and 10 mW/cm2 ）, the radiation time was six minutes per time, five times one week for continuous four weeks; the rats in control group didnt received radiation; flow cytometry was used to detect ROS activity and △ψm in splenic lymphocytes indirectly. Results： There was no statistically significant difference in ROS activity and△ψm between 2.5 mW/cm2 experimental group and control group （ P 〉 0. 05 ） ; ROS activities in 5, 10 mW/cm2 experimental groups were higher than that in control group, but △ψm lev- els were lower than that in control group, there were statistically significant differences （ P 〈 0. 05 ）, showing a dose - dependent manner. Conclusion： Long - term low - dose microwave radiation can induce increase of ROS activity and decrease of △ψm in splenic lymphocytes of pregnant rats, showing a dose - dependent manner.
出处
《中国妇幼保健》
CAS
北大核心
2013年第31期5194-5196,共3页
Maternal and Child Health Care of China
基金
吉林省卫生厅重点课题〔2012Z066〕
吉林省教育厅课题(吉教科合字[2013]第348号)
吉林省教育厅课题(吉教科合字[2012]第495号)
关键词
微波辐射
线粒体膜电位
活性氧
脾淋巴细胞
Microwave radiation
Mitochondrion membrane potential
Reactive oxygen species
Splenic lymphocyte
