摘要
目的探讨不同辐照剂量率X射线对小鼠外周血细胞及免疫器官的影响。方法 120只小鼠随机分为正常对照组、低剂量率照射组和高剂量率照射组,每组40只,雌雄各半。分别于照射后24h、48h、96h、192h每组各取10只小鼠摘眼球取血用于测定红细胞计数(RBC)、白细胞计数(WBC)、血小板数(PLT)和血红蛋白(HGB)含量,然后处死小鼠取胸腺和脾脏称重,计算胸腺指数和脾脏指数。另取一部分做组织匀浆,测定匀浆中的超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果不同剂量率X射线照射均会造成小鼠外周血细胞中RBC、WBC、PLT和HGB的降低,高、低剂量率组间无显著差异。在相同取样时间,高、低剂量率组小鼠胸腺、脾脏指数与对照组比较均差异显著,高、低剂量率组间脾脏指数差异显著,在取样时间24 h,高、低剂量率组间胸腺指数差异显著。高、低剂量率组小鼠胸腺、脾脏组织匀浆中MDA的含量均升高,SOD含量均降低,且与对照组比较差异显著。高、低剂量率组小鼠胸腺组织匀浆中MDA的含量在取样时间48 h、96 h差异显著,SOD的含量在取样时间24 h、48 h、96 h差异显著;脾脏组织匀浆中MDA的含量在取样时间24 h、48h差异显著,SOD的含量在取样时间24 h、48 h、192 h差异显著。结论高、低剂量率辐照对小鼠外周血细胞数量的影响无显著差异;高剂量率辐照对小鼠脾脏指数的影响较低剂量率大,对胸腺指数的影响无显著差异;高剂量率辐照小鼠胸腺、脾脏组织中SOD和MDA的影响较低剂量率大。
Objective To explore the effects of different irradiation dose rate of X-ray on mouse peripheral blood cells and immune organ in mouse. Methods Totally 120 mice were randomly divided into 3 groups： the normal control group, low-dose rate irradiated group and high-dose rate irradiated group. The blood samples were taken respectively from mouse of each group at 24 h, 48 h, 96 h, 192 h after irradiation for detecting red blood cells （RBC）, white blood cells （WBC）, platelets （PLT） and hemoglobins （HGB）, and the thymus glands and spleens were taken for calculating organ indexs and determining the content of superoxide dismutase （SOD） and malondialdehyde （MDA）. Result The content of RBC, WBC, PLT and HGB in mice were decreased after the different radiation dosages of X-ray, but no significant difference was observed between the high-dose group and the low-dose group. At the same sampling time, a significant difference was observed on the thymus glands index and spleens index of the high-dose and low-dose mice compared with those of the normal control mice. There was a significant difference in the spleens index of the high-dose group and the low-dose group. The thymus glands index had a significant difference between the high-dose group and the low-dose group at the 24 h after irradiation. The contents of MDA were significantly increased, and the contents of SOD were significantly decreased in thymus glands and spleens of the high- and low-dose mice compared with the normal control mice. There was a significant difference in the content of MDA of thymus glands between the high- and low-dose mouse at the sampling time of 48 h and 96 h respectively, while the SOD was observed at the sampling time of 24 h, 48 h and 96 h respectively. There was a significant difference in the content of MDA of spleens between the high- and low-dose mice at sampling time of 24 h and 48 h respectively, while the SOD was observed at the sampling time of 24 h, 48 h and 192 h respectively. Conclusion No significant difference was observed on the effect of high- and low-dose irradiation on the peripheral blood cell in mice. The high-dose irradiation had more influence than low-dose irradiation on the spleen index, while the thymus glands index was not affected by irradiation. The high-dose irradiation had more influence than the low-dose irradiation on the SOD and MDA of the thymus glands and spleens in mice.
作者
于纯淼
王贺
赵力松
孟丹
郭旭
于栋华
YU Chun-miao WANG He ZHAO Li-song MENG Dan GUO Xu YU Dong-hua(Pharmaceutical College, Heilongjiang University of Chinese Medicine, Harbin 150040, China Harbin Maternity and Child Health Care Hospital, Harbin 150040, China Department of Radiotherapy and Chemotherapy, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150040, China Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China)
出处
《实验动物与比较医学》
CAS
2017年第2期140-143,159,共5页
Laboratory Animal and Comparative Medicine
基金
黑龙江省教育厅科学技术研究面上项目(12541745)
