摘要
为动态观察兔髂动脉粥样硬化模型中不同时间点血脂和内膜的变化。将雄性新西兰白兔随机分为正常饮食组 (n=7)和高脂饮食组 (n=2 8)。用球囊导管内皮剥脱及高胆固醇饮食造成动脉粥样损伤 ,分别在内皮损伤后 1、2、4、8周及高脂饮食前取动脉血 ,然后处死动物并取损伤侧髂动脉及腹主动脉 ,常规 HE染色、电镜检查、酶法测血脂和计算机图像分析的方法进行观察。发现血清中 TC、TG、L DL-C和内膜厚度、内膜 /中膜厚度比随术后时间的延长而增加 ,动脉内膜厚度与 TC、TG、L DL-C的相关系数分别为 0 .8981、0 .8793、0 .86 82 (P<0 .0 1 )。结果提示 :该模型符合人类动脉粥样硬化的临床特征。
To investigate the changes of blood lipid and intima at different time point after atherosclerosis of iliac artery in rabbits. The New Zealand White rabbits were divided randomly into normal diet group ( n =7) and hypercholesterol diet group ( n =28). The atherosclerotic lesions were developed by inducing endothelial denudation with balloon catheter and being given high dosage of cholesterol diet at the same time. The artery blood were sampled and iliac arteries were removed at 1,2,4,8 weeks after endothelial injury respectively. The results showed that the level of TC , TG , LDL C and intimal thickness, intima/media(I/M) increased continually with the time. Coefficient of linear correlation between intimal thickness and TC、TG、LDL C were 0.8981、0.8793 and 0.8682 ( P <0.01) respectively. The results suggested that clinic character of the process of atherosclerosis in the animal model was similar to that in human.
出处
《上海实验动物科学》
2000年第4期213-216,T004,共5页
Shanghai Laboratory Animal Science
