摘要
巴戟天细胞在含ＮＡＡ０．２５ｍｇ／Ｌ，Ｋｔ０．０２５ｍｇ／Ｌ的Ｂ５培养基中进行悬浮培养，细胞比生长速率为０．１４０天－１，倍增时间为１１８．８小时，蒽醌含量可达１５１．２７ｍｇ／Ｌ，为细胞干重的０．８１％。巴戟天细胞中蒽醌含量是巴戟天根的２．１７倍，蒽醌的比生产速率是巴戟天根的７９．３倍，在固体培养愈伤组织中其蒽醌含量和比产率则分别是巴戟天根的６．１倍和２０９倍。
Cells from Morinda Officinalis How were suspension cultured on the B 5 medium containing 0.25 mg/L of NAA,0.025 mg/L of kinetin and 30g/L of sucrose.The specific growth rate of the cells was 0.14 day -1 ,the double time for the cell growth was 118 hours.The yield of anthraquinone was 151 mg/L,the content of anthraquinone in the cultured cell was 0.81 g per 100 g of dried cell weight which was 2.17 fold of that in the plant root. The specific production rate of anthraquinone in the cultured cell was 79 fold higher than that in the plant root of Morinda Officinalis How .
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1997年第2期62-67,共6页
Journal of South China University of Technology(Natural Science Edition)
基金
国家自然科学基金
关键词
植物细胞培养
蒽醌
巴戟天
plant cell culture
anthraquinone
Morinda Officinalis How
