摘要
用流式细胞术 (FCM)检测分析了激光照射对弓形虫 RH株速殖子的作用。结果显示 ,激光照射后弓形虫速殖子群体 DNA含量分布发生改变 ,DNA含量的第 1道数峰值 (平均值 )左移 ,第 1峰值分布范围内速殖子数占总速殖子数百分率增加 ,3.5 W和 4.5 W剂量照射组与对照组相比差异存在显著性 (P<0 .0 5 )。提示激光照射能抑制弓形虫 DNA的合成 ,减少弓形虫群体
The effect of laser irradiated on Toxoplasma gondii tachyzoites(RH strain) population(10 4 organisms) was analyzed by flow cytometry. The results showed that the DNA content of laser irradiated Toxoplasma changed. The peak value(mean value) of the first peak of DNA content decreased from 100 to 60, and the percentage of tachyzoite number in the first peak increased, especially 3.5 W and 4.5 W laser irradiated groups had great difference comparing with control group( P <0.05). Thus we deem that laser irradiation can inhibit the DNA synthesis, and decrease the DNA content of T. gondii tachyzoites.
出处
《中国寄生虫病防治杂志》
CSCD
2000年第3期175-177,共3页
Chinese Journal of Parasitic Disease Control
基金
浙江省卫生厅基金
关键词
激光照射
弓形体
速殖子
流式细胞仪
DNA
Laser irradiation
Toxoplasma gondii
tachyzoites
flow cytometry
DNA content
