摘要
目的建立高分离度快速液相色谱（RRLC）法同时测定蒲地蓝消炎胶囊中绿原酸、野黄芩苷、黄芩苷和黄芩素的方法。方法采用ZORBAX SB-C18柱（150 mm×4.6 mm,1.8μm）,0.4%磷酸水溶液-乙腈为流动相的梯度洗脱模式分离各测定组分,采用可变波长的方式在最大吸收波长处测定相应组分的峰面积并计算样品含量。结果绿原酸、野黄芩苷、黄芩苷和黄芩素分别在0.004330~0.4330μg（r=0.9999）、0.01740~1.740μg（r=0.9998）、0.005860~0.5860μg（r=0.9998）及0.006700~0.6700μg（r=0.9999）范围内具有良好的线性关系;且平均加样回收率和RSD分别为101.0%,2.0%;103.1%,1.7%;100.6%,0.6%;102.0%,0.7%（n=6）;重复性试验,4个成分含量的RSD均小于2.0%（n=6）。结论所建立的方法简便、快速、准确,具有良好的重复性和稳定性,可用于蒲地蓝消炎胶囊的质量控制。
Objective To establish a simultaneous method to determine the content of chlorogenic acid,scutellarin,baicalin and baicalein in Pudilan Xiaoyan Capsule by rapid resolution liquid chromatography（RRLC）.Methods A ZORBAX SB-C18 column（150 mm×4.6 mm,1.8 μm） was adopted with a gradient elution mode and a variable detecting wavelength.The mobile phase consisted of 0.4 % phosphoric acid water solution-acetonitrile.Peak area at the maximum wavelength was detected for the simultaneous determination of the components.Results Chlorogenic acid,scutellarin,baicalin and baicalein all had good linearity in the ranges of 0.004330~0.4330 μg（r=0.9999）,0.01740~ 1.740 μg（r=0.9998）,0.005860~0.5860 μg（r=0.9998）,0.006700~0.6700 μg（r=0.9999）,and the average recoveries were 101.0%（RSD=2.0 %,n=6）,103.1 %（RSD=1.7 %,n=6）,100.6 %（RSD=0.6 %,n=6）,102.0 %（RSD=0.7 %,n=6）,respectively.Repeatability experiments presented that RSD values of the four components contents were less than 2.0 %.Conclusion The established method is simple,rapid and accurate with good repeatability and stability,and is suitable for the quality control of Pudilan Xiaoyan Capsule.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2013年第4期400-403,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
蒲地蓝消炎胶囊
绿原酸
野黄芩苷
黄芩苷
黄芩素
高分离度快速液相色谱
Pudilan Xiaoyan Capsule
Chlorogenic acid
Sculellarin
Baicalin
Baicalein
Rapid resolution liquid chromatography
